How to perform Western Blot with Rabbit Poly-clonal antibodies?

SDS Page Electrophoresis of human proteins. transfer the migrated proteins on a PVDF or nitrocellulose blot. Stain the paper with rabbit poly-clonal antibodies.   The protocol of the WB  the extraction of cellular proteins from a complex mixture of intra-cellular and extracellular proteins with Biovision or Alpha Diagnostics protein extraction kit from tissues, cells, organs

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