Comparative compositional and functional analyses of Bothrops moojeni specimens reveal several individual variations.

Snake venoms are complex protein mixtures with different biological activities that can act in both their preys and human victims. Many of these proteins play a role in prey capture and in the digestive process of these animals.

It is known that some snakes are resistant to the toxicity of their own venom by mechanisms not yet fully elucidated. However, it was observed in the Laboratory of Herpetology of Instituto Butantan that some Bothrops moojeni individuals injured by the same snake species showed mortalities caused by envenoming effects. This study analyzed the biochemical composition of 13 venom and plasma samples from Bothrops moojeni specimens to assess differences in their protein composition.

Application of sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDSPAGE) showed distinct venom protein profiles, but very homogeneous plasma profiles. Western Blotting (WB) was performed with plasma samples, which were submitted to incubation with the respective venom.

Some individuals showed an immunorecognized band zone around 25 kDa, indicating interaction between the same individual plasma and venom proteins. Crossed-WB assay using non-self-plasma and venom showed that this variability is due to venom protein composition instead of plasma composition.

These venoms presented higher caseinolytic, collagenolytic and coagulant activities than the venoms without these regions recognized by WB. Mass spectrometry analyses performed on two individuals revealed that these individuals present, in addition to higher protein concentrations, other exclusive proteins in their composition. When these same two samples were tested in vivo, the results also showed higher lethality in these venoms, but lower hemorrhagic activity than in the venoms without these regions recognized by WB.

5-FOA, 25g

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Chlortetracycline HCl EP

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25G Riboflavin

NAT1058 25G
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25G PPO

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25G AquaPor LE

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25G AquaPor LM

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25G AquaPor HR

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25G Ammonium Persulfate

NAT1062 25G
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25G Alcian Blue

NAT1344 25G
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25G Biebrich Scarlet

NAT1346 25G
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25G Basic Fuchsin

NAT1350 25G
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25G Methylene Blue

NAT1352 25G
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25G Butyl PBD

NAT1510 25G
EUR 160

25G p-Terphenyl

NAT1520 25G
EUR 130

25G Bis-MSB

NAT1528 25G
EUR 187

25G AquaPor 3:1

NAT1038 25G
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25G Fast Green FCF

NAT1348 25G
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25G Amido Black 10B

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25G Xylene Cyanol FF

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AGAROSE 25G-125G -500G

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Salicin

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Oxolinic acid

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Picloram

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In conclusion, some Bothrops moojeni specimens differ in venom composition, which may have implications in envenomation. Moreover, the high individual venom variability found in this species demonstrates the importance to work with individual analyses in studies involving intraspecific venom variability and venom evolution.