Comparative compositional and functional analyses of Bothrops moojeni specimens reveal several individual variations.

Snake venoms are complex protein mixtures with different biological activities that can act in both their preys and human victims. Many of these proteins play a role in prey capture and in the digestive process of these animals.

It is known that some snakes are resistant to the toxicity of their own venom by mechanisms not yet fully elucidated. However, it was observed in the Laboratory of Herpetology of Instituto Butantan that some Bothrops moojeni individuals injured by the same snake species showed mortalities caused by envenoming effects. This study analyzed the biochemical composition of 13 venom and plasma samples from Bothrops moojeni specimens to assess differences in their protein composition.

Application of sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDSPAGE) showed distinct venom protein profiles, but very homogeneous plasma profiles. Western Blotting (WB) was performed with plasma samples, which were submitted to incubation with the respective venom.

Some individuals showed an immunorecognized band zone around 25 kDa, indicating interaction between the same individual plasma and venom proteins. Crossed-WB assay using non-self-plasma and venom showed that this variability is due to venom protein composition instead of plasma composition.

These venoms presented higher caseinolytic, collagenolytic and coagulant activities than the venoms without these regions recognized by WB. Mass spectrometry analyses performed on two individuals revealed that these individuals present, in addition to higher protein concentrations, other exclusive proteins in their composition. When these same two samples were tested in vivo, the results also showed higher lethality in these venoms, but lower hemorrhagic activity than in the venoms without these regions recognized by WB.

5-FOA, 25g

R7039-25g
EUR 963.00

Doxycycline HCl, 25g

R1059-25g
EUR 471.00

Gentamycin Sulfate, 25g

R1405-25g
EUR 190.00

Chlortetracycline HCl EP

C029-25G 25 g
EUR 141.00

25G Riboflavin

NAT1058 25G
EUR 75.00

25G PPO

NAT1498 25G
EUR 86.00

25G POPOP

NAT1524 25G
EUR 137.00

25G AquaPor LE

NAT1020 25G
EUR 101.00

25G AquaPor ES

NAT1026 25G
EUR 116.00

25G AquaPor LM

NAT1030 25G
EUR 212.00

25G AquaPor HR

NAT1034 25G
EUR 176.00

25G DATD Ultrapure

NAT1044 25G
EUR 126.00

25G Ammonium Persulfate

NAT1062 25G
EUR 75.00

25G Alcian Blue

NAT1344 25G
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25G Biebrich Scarlet

NAT1346 25G
EUR 109.00

25G Basic Fuchsin

NAT1350 25G
EUR 258.00

25G Methylene Blue

NAT1352 25G
EUR 141.00

25G Butyl PBD

NAT1510 25G
EUR 144.00

25G p-Terphenyl

NAT1520 25G
EUR 117.00

25G Bis-MSB

NAT1528 25G
EUR 167.00

AGAROSE 25G-125G -500G

A01-118-10kg 10 kg Ask for price

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A01-118-2kg 2kg Ask for price

AGAROSE 25G-125G -500G

A01-118-500g 500 g
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NAT1038 25G
EUR 185.00

25G Fast Green FCF

NAT1348 25G
EUR 144.00

25G Amido Black 10B

NAT1354 25G
EUR 133.00

25G Xylene Cyanol FF

NAT1368 25G
EUR 172.00

25G Bis (Methylene Bis-Acrylamide)

NAT1042 25G
EUR 79.00

Chlortetracycline HCl EP

C029-100G 100 g
EUR 321.00

Chlortetracycline HCl EP

C029-5G 5 g
EUR 65.00

DiagPoly Protein A Conjugated Polystyrene Particles, 3.0-3.9 µm

DNM-C029 5 mL
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DNG-C029 10 mL
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Malachite green oxalate

abx180007-25g 25 g
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C.I.Basic Violet 11:1

abx180051-25g 25 g
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Iodotrimethylsilane

abx180058-25g 25 g
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4-Hydroxycyclohexanone

abx180074-25g 25 g
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2-Isonitrosoacetophenone

abx180344-25g 25 g
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abx180443-25g 25 g
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abx180502-25g 25 g
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abx180561-25g 25 g
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4-Nitrothiophenol

abx180563-25g 25 g
EUR 495.00
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5-Bromopyrimidin-4-ol

abx180608-25g 25 g
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abx180658-25g 25 g
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abx180826-25g 25 g
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abx180834-25g 25 g
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abx180913-25g 25 g
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abx180975-25g 25 g
EUR 523.00
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2-Amino-4-Chlorothiophenol

abx181014-25g 25 g
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abx181083-25g 25 g
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3-Butenenitrile

abx181104-25g 25 g
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abx181145-25g 25 g
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abx181200-25g 25 g
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Diphenyl phthalate

abx181266-25g 25 g
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Lithium tetraborate

abx181305-25g 25 g
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abx181320-25g 25 g
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abx181342-25g 25 g
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abx181500-25g 25 g
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abx181580-25g 25 g
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abx181607-25g 25 g
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5-Methylcytosine

abx185291-25g 25 g
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abx185309-25g 25 g
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abx185345-25g 25 g
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Butyl isocyanate

abx185367-25g 25 g
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C.I.Solvent Green 3

abx185369-25g 25 g
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abx185538-25g 25 g
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abx185540-25g 25 g
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Pigment Red 48

abx185541-25g 25 g
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Pigment red 49

abx185542-25g 25 g
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Pigment Red 57

abx185543-25g 25 g
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abx185545-25g 25 g
EUR 439.00
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2,2',4,4'-Tetrehydroxybenzophenone

abx185702-25g 25 g
EUR 230.00
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abx182984-25g 25 g
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abx183034-25g 25 g
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2,6-Difluorobenzylchloride

abx183035-25g 25 g
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abx183086-25g 25 g
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2-Bromophenol

abx183129-25g 25 g
EUR 230.00
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2-Norbornanemethanol

abx183253-25g 25 g
EUR 384.00
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3,5-Dimethylisoxazole-4-Boronic Acid

abx183322-25g 25 g
EUR 272.00
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abx183349-25g 25 g
EUR 314.00
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abx183380-25g 25 g
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3-Chlorophenol

abx183392-25g 25 g
EUR 203.00
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4-Chlorobenzaldehyde

abx183575-25g 25 g
EUR 203.00
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4-Methoxybenzoyl chloride

abx183621-25g 25 g
EUR 217.00
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4-Piperidone

abx183645-25g 25 g
EUR 314.00
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5-Bromo-2,2-difluoro-1,3-benzodioxole

abx183672-25g 25 g
EUR 565.00
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Lithium oxide

abx184716-25g 25 g
EUR 203.00
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Methyl-3,3dimethyl acrylate

abx184732-25g 25 g
EUR 342.00
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Neodymium fluoride(NdF3)

abx184738-25g 25 g
EUR 217.00
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Sulfamethazine

abx184776-25g 25 g
EUR 189.00
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Testosterone enanthate

abx184781-25g 25 g
EUR 481.00
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Trimethoprim

abx184794-25g 25 g
EUR 578.00
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(2R)-2-[(4-Ethyl-2,3-dioxopiperazinyl)carbonylamino]-2-(4-hydroxyphenyl)acetic acid

abx182726-25g 25 g
EUR 272.00
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(R)-(-)-Epichlorohydrin

abx182749-25g 25 g
EUR 203.00
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(R)-2-Aminobutan-1-ol

abx182758-25g 25 g
EUR 217.00
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(S)-4-N-Boc-2-methylpiperazine

abx182784-25g 25 g
EUR 286.00
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1-Hydroxybenzotriazole

abx182888-25g 25 g
EUR 217.00
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1-N-BOC-Azetidine-3-carboxylic acid

abx182907-25g 25 g
EUR 286.00
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C.I.Var Blue 6

abx180699-25g 25 g
EUR 551.00
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2-Amino-3-fluoro-5-trifluoromethylpyridine

abx181678-25g 25 g
EUR 718.00
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2-Bromopyridine-5-carbaldehyde

abx181744-25g 25 g
EUR 425.00
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3-Aminopicolinic acid

abx181893-25g 25 g
EUR 384.00
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3-Azetidinecarboxylic acid

abx181898-25g 25 g
EUR 314.00
  • Shipped within 1-2 weeks.

3-Bromoisonicotinic acid

abx181922-25g 25 g
EUR 230.00
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Methyl 1H-pyrazole-3-carboxylate

abx182518-25g 25 g
EUR 342.00
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Methyl indole-4-carboxylate

abx182565-25g 25 g
EUR 314.00
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tert-Butyl 4-(4,4,5,5-tetramethyl-1,3,2-dioxaborol-an-2-yl)-5,6-dihydropyridine-1(2H)-carboxylate

abx182663-25g 25 g
EUR 634.00
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Nonadecanedioic Acid

abx188823-25g 25 g
EUR 1358.00
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S-23

abx188871-25g 25 g
EUR 732.00
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Tert-Butyl (R)-2-Oxopiperidin-3-Ylcarbamate

abx188899-25g 25 g
EUR 1706.00
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Tianeptine Sodium Salt

abx188914-25g 25 g
EUR 314.00
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Trans-2-(3-Fluorophenyl)Vinylboronic Acid

abx188916-25g 25 g
EUR 1720.00
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Trans-2-[4-(Trifluoromethyl)Phenyl]Vinylboronic Acid

abx188917-25g 25 g
EUR 1720.00
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L-Proline

abx082114-25g 25 g
EUR 175.00
  • Shipped within 5-10 working days.

D-(+)-Galactose

abx082116-25g 25 g
EUR 189.00
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Sephadex G-100

abx082118-25g 25 g
EUR 439.00
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Sephadex G-50

abx082119-25g 25 g
EUR 342.00
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Sephadex G-25

abx082120-25g 25 g
EUR 342.00
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D-Mannitol

abx082121-25g 25 g
EUR 217.00
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3-Indoleacetic acid

abx082125-25g 25 g
EUR 230.00
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L-ArginineHCL

abx082127-25g 25 g
EUR 189.00
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D-(+)-Trehalose dihydrate

abx082139-25g 25 g
EUR 217.00
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L-Serine

abx082142-25g 25 g
EUR 189.00
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D-(+)-Galactose

abx082291-25g 25 g
EUR 203.00
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L-ArginineHCL

abx082294-25g 25 g
EUR 189.00
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L-Methionine

abx082295-25g 25 g
EUR 189.00
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Pyridoxine hydrochloride

abx082298-25g 25 g
EUR 217.00
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L-Lysine HCL

abx082304-25g 25 g
EUR 175.00
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TES

abx082351-25g 25 g
EUR 217.00
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Pepsin

abx082440-25g 25 g
EUR 189.00
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D-(+)-Galactose

abx082441-25g 25 g
EUR 189.00
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Albumin from chicken egg white

abx082474-25g 25 g
EUR 189.00
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Bis-Acrylamide

abx082552-25g 25 g
EUR 175.00
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L-Proline

abx082581-25g 25 g
EUR 189.00
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N-Acety-L-Cysteine

abx082583-25g 25 g
EUR 203.00
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5-Methylindole

abx183751-25g 25 g
EUR 398.00
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7-(4-Bromobutoxy)-3,4-dihydro-2(1H)-quinolinone

abx183806-25g 25 g
EUR 592.00
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Allyl chloroformate

abx183843-25g 25 g
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Antazoline HCl

abx183853-25g 25 g
EUR 286.00
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Azure B

abx183860-25g 25 g
EUR 217.00
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C.I.Solvent Red 23

abx183922-25g 25 g
EUR 217.00
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Ceftriaxone sodium

abx183931-25g 25 g
EUR 272.00
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N-Boc-cis-4-Hydroxy-L-proline methyl ester

abx184269-25g 25 g
EUR 328.00
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N-Methylcyclohexylamine

abx184283-25g 25 g
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Testosterone propionate

abx184411-25g 25 g
EUR 578.00
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abx184419-25g 25 g
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Theophylline

abx184435-25g 25 g
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abx184463-25g 25 g
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Triphosgene

abx184464-25g 25 g
EUR 217.00
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(S)-N-Glycidylphthalimide

abx184516-25g 25 g
EUR 230.00
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1,1,1,-Trichloro-2,2,2-Trifluoroethane

abx184522-25g 25 g
EUR 286.00
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6,7-Dimethoxy-1H-quinazolin-4-one

abx184629-25g 25 g
EUR 258.00
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Calcium β-hydroxy-β-methylbutyric acid

abx184656-25g 25 g
EUR 230.00
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Nystatin

abx186017-25g 25 g
EUR 411.00
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1-(2-Furoyl)piperazine

abx186106-25g 25 g
EUR 272.00
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1,3-Bis(isocyanatomethyl)benzene

abx186124-25g 25 g
EUR 272.00
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2,3-Dibromo-5-(Trifluoromethyl)Pyridine

abx186177-25g 25 g
EUR 453.00
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3-Fluorophenethyl Bromide

abx186268-25g 25 g
EUR 578.00
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3-Methyl-2-butanone

abx186274-25g 25 g
EUR 189.00
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3-nitrobiphenyl

abx186279-25g 25 g
EUR 481.00
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abx186500-25g 25 g
EUR 244.00
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Methyl Sorbate

abx186506-25g 25 g
EUR 370.00
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1,4-Diazabicyclo[3.3.2]Nonane 2HCL

abx187873-25g 25 g
EUR 2555.00
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2,6-Diamine-3,5-Dibromopyridine

abx187883-25g 25 g
EUR 1177.00
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Methyl 2-Formylthiazole-4-Carboxylate

abx187932-25g 25 g
EUR 3474.00
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Potassium 2-Methoxyethoxymethyltrifluoroborate

abx187937-25g 25 g
EUR 2388.00
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Tert-Butyl ((3-Hydroxyazetidin-3-Yl)Methyl)Carbamate

abx187942-25g 25 g
EUR 3056.00
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(5-Bromo-2-Chloropyridin-4-Yl)Methanol

abx188016-25g 25 g
EUR 1149.00
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(Dimethylphenylsilyl)Boronic Acid Pinac&

abx188018-25g 25 g
EUR 871.00
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(S)-Allyl 2-((((9H-Fluoren-9-Yl)Methoxy)Carbonyl)Amino)-6-Aminohexanoate Hydrochloride

abx188038-25g 25 g
EUR 1219.00
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(S)-N-[1-(2-Fluorophenyl)Ethyl]Methylamine

abx188040-25g 25 g
EUR 3098.00
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[1,2,4]Triazolo[1,5-A]Pyridine-7-Carboxylicacid

abx188046-25g 25 g
EUR 2973.00
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1-(2-Bromoethyl)Piperidine Hydrobromide

abx188070-25g 25 g
EUR 913.00
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1,12-Dibromododecane

abx188079-25g 25 g
EUR 467.00
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1,2-Dihydro-2,2,4-Trimethylquinoline

abx188088-25g 25 g
EUR 286.00
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1-Boc-4-(4-Bromopyrazol-1-Yl)Piperidine

abx188116-25g 25 g
EUR 467.00
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2-(2,2-Dimethoxyethyl) Benzamine

abx188144-25g 25 g
EUR 2068.00
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2-(4-Fluoro-3-Methylphenyl)-5,5-Dimethyl-1,3,2-Dioxaborinane

abx188150-25g 25 g
EUR 1038.00
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2-(Aminomethyl)-1,3-Dioxolane

abx188152-25g 25 g
EUR 871.00
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2-(N-Morpholino)Ethanesulfonic Acid Monohydrate,

abx188157-25g 25 g
EUR 314.00
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2-Chloroquinolin-7-Ol

abx188234-25g 25 g
EUR 1400.00
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2-Cyanotetrahydrofuran

abx188238-25g 25 g
EUR 1497.00
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2-Deoxy-2-Fluoro-Alpha-D-Arabinofuranosyl Bromide 3,5-Dibenzoate

abx188240-25g 25 g
EUR 2179.00
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2-Methyl-1H-Imidazole-4-Carboxylic Acid

abx188264-25g 25 g
EUR 1386.00
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2-N-Boc-Butane-1,2-Diamine Hydrochloride

abx188273-25g 25 g
EUR 2555.00
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2-Oxindole-3-Acetic Acid

abx188274-25g 25 g
EUR 1358.00
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3-(4-Hydroxy-3,5-Diiodo)-Benzoyl-2-Butyl Benzofuran

abx188297-25g 25 g
EUR 314.00
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3-(Pyrrolidin-1-Ylmethyl)Benzoic Acid Hydrochloride

abx188312-25g 25 g
EUR 1469.00
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3,4,5-Trihydroxybenzaldehyde Hydrate

abx188319-25g 25 g
EUR 1191.00
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3,4-Dimethoxyphenylhydrazine Hydrochloride

abx188329-25g 25 g
EUR 523.00
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3,6-Dihydro-2H-Pyran-4-Carbonitrile

abx188341-25g 25 g
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In conclusion, some Bothrops moojeni specimens differ in venom composition, which may have implications in envenomation. Moreover, the high individual venom variability found in this species demonstrates the importance to work with individual analyses in studies involving intraspecific venom variability and venom evolution.

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